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KMID : 0380619720040020123
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Korean Journal of Food Science and Technology
1972 Volume.4 No. 2 p.123 ~ p.133
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Studies on the Production of Lysine by Fermentation Process(2)
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권태완/Kwon, Tai Wan
민태익/김항묵/Mheen, Tae Ick/Kim, Hang Mook
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Abstract
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Over 90 of lysine producing auxotrophs were obtained from Corynebacterium sp. S-27-12, Brevibacterium flavum ATCC 15168 and Micrococcus glutamicus ATCC 13032 by UV light, Co^(6´) irradiation and N-methyl-N´-nitro-N-nitrosoguanidine treatment.
One of the mutant, Brev. flavum U46-N59, was identified as a leucine auxotroph and accumulated lysine during flask (500 ㎖) cultivation (180 strokes/min.) up to 21.6 ㎎ per ㎖ of broth at pH 7.5 and 28℃ after 4 days. The medium consisted of glucose, 100; urea, 10; corn steep liquor, 40; KH₂PO₄ 2; K₂HPO₄, 0.5; MgSO₄. 7H₂O, 0.4; antifoam S-57, 1 g; Fe₂(SO₄)₃.XH₂O, 10; MnCl₂, 4H₂O, 10 ㎎; biotin, 30; thiamine-HCl, 100 ㎍ in 1ℓ of distilled water, and 40 U/㎖ of penicillin was added after 36 hrs fermentation.
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